Defective dendritic cell response to Toll-like receptor 7/8 agonists in perinatally HIV-infected children

Synergy between Vitamin D3 and Toll-Like Receptor Agonists Regulates Human Dendritic Cell Response during Maturation

Human dendritic cells (DC) can be differentiated from blood monocytes in the presence of GM-CSF and IL-4 and matured by lipopolysaccharide (LPS). Vitamin D3 inhibits the maturation of human DC measured by changes in surface expression of HLA-DR, CD14, CD40, CD80, CD83, and CD86. We here examine the function of vitamin D3 during DC maturation. One of the earliest changes to LPS-induced maturatio...

Hearing sensitivity in perinatally HIV-infected children

Materials and methods Children were recruited from outpatient clinics within Tygerberg Children’s Hospital in Cape Town, South Africa. Caregiver report of middle ear infections was obtained through a questionnaire. Bilateral otoscopy, tympanometry, then pure-tone testing was completed. Hearing loss was defined as a pure-tone average (PTA) of 500, 1000, 2000, and 4000 Hz of >15 decibels of heari...

Natural toll-like receptor agonists in prophylactic vaccines for dendritic cell activation

Toll-like receptor agonists as third signals for dendritic cell-tumor fusion vaccines.

BACKGROUND The aim of the present study was to evaluate the therapeutic efficacy of dendritic cell (DC)-tumor fusion hybrids with Toll-like receptor (TLR) agonists. METHODS DC-tumor fusion hybrids were generated by electrofusion and injected into the inguinal lymph nodes of C57BL/6 mice with 3-day established pulmonary metastases. Paired TLR agonists polyinosine:polycytadilic acid [poly(I:C)]...

Properties regulating the nature of the plasmacytoid dendritic cell response to Toll-like receptor 9 activation

Human plasmacytoid dendritic cells (PDCs) can produce interferon (IFN)-alpha and/or mature and participate in the adaptive immune response. Three classes of CpG oligonucleotide ligands for Toll-like receptor (TLR)9 can be distinguished by different sequence motifs and different abilities to stimulate IFN-alpha production and maturation of PDCs. We show that the nature of the PDC response is det...